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Benchling Inc crispr guide rna design tool
Crispr Guide Rna Design Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispr+guide+rna+design+tool/crispr+design+tool/pmc13155868-62-34-40
Average 86 stars, based on 1 article reviews
crispr guide rna design tool - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

CRISPR:

Article Title: Reduced Maintenance DNA Methylation Thresholds Enable Sensitive Reporter Assays for UHRF1 and DNMT1 Inhibition.
Article Snippet: Short hairpin RNA (shRNA) sequences targeting UHRF1 were cloned into pLKO.1 (Addgene, 10878). .. Single guide RNAs (sgRNAs) were designed with the Benchling CRISPR Guide RNA Design tool ( https://www.benchling.com/crispr ) and subcloned into pX330 (Addgene, 110403). shRNA and sgRNA primer sequences are listed in Table S3 . ..

Article Title: IDH-mutant gliomas arise from glial progenitor cells harboring the initial driver mutation.
Article Snippet: INTRODUCTION: Isocitrate dehydrogenase (IDH)–mutant gliomas are the most common malignant brain tumors in adults under age 50, comprising two subtypes, oligodendroglioma with 1p/19q codeletion and astrocytoma that do not harbor the 1p/19q codeletion.. Previous transcriptomic analyses have revealed shared glial lineages across both subtypes, suggesting a common progenitor cell.. The IDH mutation is thought to occur early in glioma development, but whether it is the first driver event and which brain cell type serves as the cell of origin remain unclear.

Article Title: Treatment of Huntington's disease with a pan-HTT-targeting CRISPR nuclease.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: Treatment of Huntington’s disease with a pan-HTT-targeting CRISPR nuclease
Article Snippet: .. All primer sequences for constructing the Cas9 variants are provided in Table S3 . sgRNAs for HTT exon 1 were identified by the CRISPR Guide RNA Design Tool in Benchling. ..

Article Title: Ligustroflavone protects against acute kidney injury by inhibiting ferroptosis via acting on GSK3β/NRF2 signaling
Article Snippet: .. GSK3β-knockout (GSK3β-KO) TKPTs constructed: The CRISPR/Cas9 plasmids targeting mouse GSK3β [the guide (g) RNA sequences are presented in ] were constructed by using PX459 (cat. no. 62988; Addgene, Inc.). gRNAs were designed using Benchling's CRISPR Guide RNA Design Tool ( https://benchling.com/ ). gRNA1 targeted exon 1 of GSK3b, affecting the N-terminal regulatory region, while gRNA2 targeted exon 5, affecting the C-terminal catalytic lobe. .. Then the plasmids were transfected into TKPTs using Lipofectamine 2000 (cat. no. 11,668,030; Thermo Fisher Scientific, Inc.), and the positive cells were selected using puromycin (2 μ g/ml) for 3 days prior to clonal expansion.

Article Title: Deciphering strain differences in codY regulation of Clostridioides difficile s sporulation
Article Snippet: .. To generate sgRNAs, the Benchling CRISPR Guide RNA Design tool was used. sgRNAs were amplified by PCR and cloned into pMC1123 ( , ). ..

Article Title: Generation and validation of a human iPSC-derived TDP-43 knockout model for ALS disease modeling
Article Snippet: .. sgRNAs targeting exon 1 or exon 2 of TARDBP were designed using Benchling’s CRISPR guide RNA design tool and selected based on predicted high on-target efficiency and low off-target activity. .. Synthetic chemically modified sgRNAs were purchased from Synthego, resuspended in TE buffer to a final concentration of 100 μM, and stored at −80 °C until use.

Article Title: Reduced mediodorsal thalamus activity underlies aberrant belief dynamics in a genetic mouse model of schizophrenia.
Article Snippet: .. CRISPR (cr)RNAs were designed using the CRISPR Guide RNA Design Tool (https://www.benchling.com/crispr) and tested by SURVEYOR assay (Transgenomic) according to the manufacturer’s protocol. ..

shRNA:

Article Title: Reduced Maintenance DNA Methylation Thresholds Enable Sensitive Reporter Assays for UHRF1 and DNMT1 Inhibition.
Article Snippet: Short hairpin RNA (shRNA) sequences targeting UHRF1 were cloned into pLKO.1 (Addgene, 10878). .. Single guide RNAs (sgRNAs) were designed with the Benchling CRISPR Guide RNA Design tool ( https://www.benchling.com/crispr ) and subcloned into pX330 (Addgene, 110403). shRNA and sgRNA primer sequences are listed in Table S3 . ..

Sequencing:

Article Title: IDH-mutant gliomas arise from glial progenitor cells harboring the initial driver mutation.
Article Snippet: INTRODUCTION: Isocitrate dehydrogenase (IDH)–mutant gliomas are the most common malignant brain tumors in adults under age 50, comprising two subtypes, oligodendroglioma with 1p/19q codeletion and astrocytoma that do not harbor the 1p/19q codeletion.. Previous transcriptomic analyses have revealed shared glial lineages across both subtypes, suggesting a common progenitor cell.. The IDH mutation is thought to occur early in glioma development, but whether it is the first driver event and which brain cell type serves as the cell of origin remain unclear.

Construct:

Article Title: Ligustroflavone protects against acute kidney injury by inhibiting ferroptosis via acting on GSK3β/NRF2 signaling
Article Snippet: .. GSK3β-knockout (GSK3β-KO) TKPTs constructed: The CRISPR/Cas9 plasmids targeting mouse GSK3β [the guide (g) RNA sequences are presented in ] were constructed by using PX459 (cat. no. 62988; Addgene, Inc.). gRNAs were designed using Benchling's CRISPR Guide RNA Design Tool ( https://benchling.com/ ). gRNA1 targeted exon 1 of GSK3b, affecting the N-terminal regulatory region, while gRNA2 targeted exon 5, affecting the C-terminal catalytic lobe. .. Then the plasmids were transfected into TKPTs using Lipofectamine 2000 (cat. no. 11,668,030; Thermo Fisher Scientific, Inc.), and the positive cells were selected using puromycin (2 μ g/ml) for 3 days prior to clonal expansion.

Amplification:

Article Title: Deciphering strain differences in codY regulation of Clostridioides difficile s sporulation
Article Snippet: .. To generate sgRNAs, the Benchling CRISPR Guide RNA Design tool was used. sgRNAs were amplified by PCR and cloned into pMC1123 ( , ). ..

Polymerase Chain Reaction:

Article Title: Deciphering strain differences in codY regulation of Clostridioides difficile s sporulation
Article Snippet: .. To generate sgRNAs, the Benchling CRISPR Guide RNA Design tool was used. sgRNAs were amplified by PCR and cloned into pMC1123 ( , ). ..

Clone Assay:

Article Title: Deciphering strain differences in codY regulation of Clostridioides difficile s sporulation
Article Snippet: .. To generate sgRNAs, the Benchling CRISPR Guide RNA Design tool was used. sgRNAs were amplified by PCR and cloned into pMC1123 ( , ). ..

Activity Assay:

Article Title: Generation and validation of a human iPSC-derived TDP-43 knockout model for ALS disease modeling
Article Snippet: .. sgRNAs targeting exon 1 or exon 2 of TARDBP were designed using Benchling’s CRISPR guide RNA design tool and selected based on predicted high on-target efficiency and low off-target activity. .. Synthetic chemically modified sgRNAs were purchased from Synthego, resuspended in TE buffer to a final concentration of 100 μM, and stored at −80 °C until use.



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